Linked Life Data

20560137

Source:http://linkedlifedata.com/resource/pubmed/id/20560137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2011-2-23
pubmed:abstractText
Although adjuvant chemotherapy has significantly increased overall survival in resected Stage III colorectal cancer, disease recurrence is still high (30-40%). 20-25% of Stage II patients also develop recurrent disease. Thus, high-risk patients may benefit from chemotherapy. As patient response to standard chemotherapy varies, the study of molecular differences in the expression of pharmacologically relevant genes may help clinicians to understand variability and tailor therapy. The expression of 5-fluorouracil (5-FU) pathway genes in tumors from 53 Stages II-III colorectal cancer patients who underwent 5-FU adjuvant chemotherapy was investigated by reverse transcription quantitative real-time polymerase chain reaction. Patients were dichotomized into high- and low-mRNA expression level groups using median values of gene mRNA levels. Then, a threshold analysis to identify a cut-off distinguishing recurrent- or nonrecurrent-disease was used. A high degree of interpatient variation in relative tumor expression of study genes was observed. Multiple gene correlations were found, which suggest possible coregulation mechanisms. No statistically significant relationship between experimental data and baseline clinical/pathological characteristics or clinical outcome was observed using gene expression median values. Threshold analysis indicated significant inverse relationships between deoxyuridine triphosphatase (DUT), ferrodoxin reductase (FDXR) or tumor protein p53 (TP53) and disease-free survival (DFS) in the entire case series and between DUT or NM23-H1 and DFS in Stage III patients: higher gene expression was associated with shorter DFS. This study provides data on relationships between expression of 5-FU pathway genes and clinical outcome of colorectal cancer patients undergoing 5-FU adjuvant chemotherapy and underscores the predictive role of specific genes. Validation in an independent case series is warranted.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1097-0215
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 UICC.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1935-45
pubmed:meshHeading
pubmed-meshheading:20560137-Adenocarcinoma, pubmed-meshheading:20560137-Adult, pubmed-meshheading:20560137-Aged, pubmed-meshheading:20560137-Antimetabolites, Antineoplastic, pubmed-meshheading:20560137-Chemotherapy, Adjuvant, pubmed-meshheading:20560137-Colorectal Neoplasms, pubmed-meshheading:20560137-Female, pubmed-meshheading:20560137-Fluorouracil, pubmed-meshheading:20560137-Gene Expression Profiling, pubmed-meshheading:20560137-Humans, pubmed-meshheading:20560137-Male, pubmed-meshheading:20560137-Middle Aged, pubmed-meshheading:20560137-NM23 Nucleoside Diphosphate Kinases, pubmed-meshheading:20560137-Neoplasm Recurrence, Local, pubmed-meshheading:20560137-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:20560137-Pharmacogenetics, pubmed-meshheading:20560137-Prognosis, pubmed-meshheading:20560137-Pyrophosphatases, pubmed-meshheading:20560137-RNA, Messenger, pubmed-meshheading:20560137-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20560137-Survival Rate, pubmed-meshheading:20560137-Tumor Markers, Biological, pubmed-meshheading:20560137-Tumor Suppressor Protein p53
pubmed:year
2011
pubmed:articleTitle
Identification of potential pharmacogenomic markers of clinical efficacy of 5-fluorouracil in colorectal cancer.
pubmed:affiliation
Department of Pharmacology, University of Florence, Florence, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't