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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2010-7-2
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pubmed:abstractText |
Overexpression of the human epidermal growth factor receptor type 2 (HER2) in breast carcinomas predicts response to trastuzumab therapy. Affibody molecules based on a nonimmunoglobulin scaffold have demonstrated a high potential for in vivo molecular imaging of HER2-expressing tumors. The reengineering of the molecular scaffold has led to a second generation of optimized Affibody molecules that have a surface distinctly different from the parental protein domain from staphylococcal protein A. Compared with the parental molecule, the new tracer showed a further increased melting point, stability, and overall hydrophilicity and was more amenable to chemical peptide synthesis. The goal of this study was to assess the potential effects of this extensive reengineering on HER2 targeting, using ABY-025, a DOTA-conjugated variant of the novel tracer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1535-5667
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1131-8
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pubmed:meshHeading |
pubmed-meshheading:20554729-Amino Acid Sequence,
pubmed-meshheading:20554729-Amino Acids,
pubmed-meshheading:20554729-Animals,
pubmed-meshheading:20554729-Breast Neoplasms,
pubmed-meshheading:20554729-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20554729-Female,
pubmed-meshheading:20554729-Gamma Cameras,
pubmed-meshheading:20554729-Humans,
pubmed-meshheading:20554729-Immunochemistry,
pubmed-meshheading:20554729-Indium Radioisotopes,
pubmed-meshheading:20554729-Isotope Labeling,
pubmed-meshheading:20554729-Macaca fascicularis,
pubmed-meshheading:20554729-Mice,
pubmed-meshheading:20554729-Mice, Inbred BALB C,
pubmed-meshheading:20554729-Molecular Sequence Data,
pubmed-meshheading:20554729-Neoplasm Transplantation,
pubmed-meshheading:20554729-Peptide Fragments,
pubmed-meshheading:20554729-Radiopharmaceuticals,
pubmed-meshheading:20554729-Rats,
pubmed-meshheading:20554729-Rats, Sprague-Dawley,
pubmed-meshheading:20554729-Receptor, erbB-2,
pubmed-meshheading:20554729-Staphylococcal Protein A,
pubmed-meshheading:20554729-Tissue Distribution
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pubmed:year |
2010
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pubmed:articleTitle |
Targeting of HER2-expressing tumors using 111In-ABY-025, a second-generation affibody molecule with a fundamentally reengineered scaffold.
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pubmed:affiliation |
Division of Nuclear Medicine, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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