Linked Life Data

20547333

Source:http://linkedlifedata.com/resource/pubmed/id/20547333

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-8-9
pubmed:abstractText
In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7x10(7) cells/cm(3), being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1347-4421
pubmed:author
pubmed:copyrightInfo
Copyright 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
363-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes.
pubmed:affiliation
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
pubmed:publicationType
Journal Article