Linked Life Data

20545637

Source:http://linkedlifedata.com/resource/pubmed/id/20545637

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-7-14
pubmed:abstractText
During skeletogenesis, the development of a new vascular network, i.e. angiogenesis, is triggered by hypoxia through the activation of the hypoxia inducible factors (HIFs) HIF-1alpha and HIF-2alpha. HIFs regulate the expression of several genes, including those coding for angiogenic growth factors such as VEGFA, angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2). The expression of HIFs and angiogenic growth factors is well documented in endochondral ossification, but few data exist on their expression during intramembranous ossification. In this study, the localization of these factors was examined using immunohistochemistry and RT-PCR in bones of porcine foetuses. Immunostaining for HIF-1alpha and HIF-2alpha was observed during endochondral ossification, whereas only HIF-2alpha was present at sites of intramembranous ossification. Furthermore, immunostaining for ANGPT2 was present at sites of endochondral and intramembranous ossification. In addition, gene transcripts for ANGPT1, ANGPT2 and VEGFA were detected with RT-PCR in laser capture microdissected isolates from both types of ossification. These results indicate that angiogenesis plays an important role during endochondral and intramembranous ossification. However, the different expression pattern of the HIF-alpha subunits suggests that alternative regulatory pathways trigger angiogenesis in these distinct types of ossification.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1439-0264
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
376-84
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Detection of hypoxia inducible factors and angiogenic growth factors during foetal endochondral and intramembranous ossification.
pubmed:affiliation
Faculty of Veterinary Medicine, Department of Morphology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Ward.DeSpiegelaere@UGent.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't