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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-7-26
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pubmed:abstractText |
We report the 1.77-A resolution X-ray crystal structure of a dodecamer DNA duplex with the sequence 5'-CCTCTGGTCTCC-3' that has been modified to contain a single engineered 1,2-cis-{Pt(NH(3))(2)}(2+)-d(GpG) cross-link, the major DNA adduct of cisplatin. These data represent a significant improvement in resolution over the previously published 2.6-A structure. The ammine ligands in this structure are clearly resolved, leading to improved visualization of the cross-link geometry with respect to both the platinum center and to the nucleobases, which adopt a higher energy conformation. Also better resolved are the deoxyribose sugar puckers, which allow us to re-examine the global structure of platinum-modified DNA. Another new feature of this model is the location of four octahedral [Mg(H(2)O)(6)](2+) ions associated with bases in the DNA major groove and the identification of 124 ordered water molecules that participate in hydrogen-bonding interactions with either the nucleic acid or the diammineplatinum(II) moiety.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-10101191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-10320349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-10385126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-10688897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-10749874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-11416174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-11456962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-11599959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-12598364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-12930962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-14287410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-16729290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-17625587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-18579768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-2767054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-5782119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-7566180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-7687065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-8312469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-8527670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-9097732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20541266-9405373
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1873-3344
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pubmed:author |
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pubmed:copyrightInfo |
Copyright (c) 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
902-8
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pubmed:dateRevised |
2011-9-13
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Structure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}2+-d(GpG) cross-link at 1.77 A resolution.
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pubmed:affiliation |
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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