Linked Life Data

20540970

Source:http://linkedlifedata.com/resource/pubmed/id/20540970

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-8-23
pubmed:databankReference
pubmed:abstractText
Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the nuclear factor-kappa B (NF-kappaB) is activated in schizont-infected cells, making T. parva-transformed cells resistant to apoptosis. However, the mechanism by which the parasite triggers the activation of NF-kappaB remains enigmatic. In the present study, we biochemically characterized a novel protein, which we termed TpSCOP (T. parvaschizont-derived cytoskeleton-binding protein), which is expressed in the schizont stage of T. parva. TpSCOP was shown to interact with F-actin in vitro. Expression of TpSCOP in a murine lymphocytic cell line resulted in the activation of NF-kappaB signaling pathways, leading to apoptosis resistance. The activation of mitogen-activated protein kinase (MAPK), including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), was also detected. Furthermore, the introduction of TpSCOP into T. parva-infected cells also enhanced the activation of NF-kappaB. This is the first report to demonstrate that a parasite-derived molecule has the ability to activate the host NF-kappaB pathway. Based on these results, TpSCOP likely plays an important role in apoptosis inhibition during Theileria infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1872-9428
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8-17
pubmed:meshHeading
pubmed-meshheading:20540970-Actins, pubmed-meshheading:20540970-Animals, pubmed-meshheading:20540970-Cell Line, pubmed-meshheading:20540970-DNA, Protozoan, pubmed-meshheading:20540970-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:20540970-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:20540970-Lymphocytes, pubmed-meshheading:20540970-Mice, pubmed-meshheading:20540970-Mitogen-Activated Protein Kinases, pubmed-meshheading:20540970-Molecular Sequence Data, pubmed-meshheading:20540970-NF-kappa B p50 Subunit, pubmed-meshheading:20540970-Protein Binding, pubmed-meshheading:20540970-Protein Interaction Mapping, pubmed-meshheading:20540970-Protozoan Proteins, pubmed-meshheading:20540970-Schizonts, pubmed-meshheading:20540970-Sequence Analysis, DNA, pubmed-meshheading:20540970-Signal Transduction, pubmed-meshheading:20540970-Theileria parva
pubmed:year
2010
pubmed:articleTitle
A schizont-derived protein, TpSCOP, is involved in the activation of NF-kappaB in Theileria parva-infected lymphocytes.
pubmed:affiliation
Department of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't