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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1520-4995
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
6
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5405-7
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
pubmed-meshheading:20540494-Azo Compounds,
pubmed-meshheading:20540494-Bacteriological Techniques,
pubmed-meshheading:20540494-Cloning, Molecular,
pubmed-meshheading:20540494-Coloring Agents,
pubmed-meshheading:20540494-Escherichia coli,
pubmed-meshheading:20540494-Gene Expression Regulation,
pubmed-meshheading:20540494-Indicators and Reagents,
pubmed-meshheading:20540494-Isopropyl Thiogalactoside,
pubmed-meshheading:20540494-NADH, NADPH Oxidoreductases,
pubmed-meshheading:20540494-Recombinant Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Look and see if it is time to induce protein expression in Escherichia coli cultures.
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pubmed:affiliation |
Department of Biochemistry and Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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