20534450

pubmed:Citation

24

Natural killer-22 (NK-22) cells are a human NK cell subset situated in mucosal-associated lymphoid tissues that specialize in IL-22 secretion in response to IL-23. Here we investigated the cytokine requirements for NK-22 cell expansion. IL-7 maintained the survival of NK-22 cells and IL-22 production in response to IL-23 but was insufficient to induce robust expansion. Proliferation of NK-22 cells was increased markedly by adding either IL-1beta or IL-2 to IL-7 and was even stronger in the presence of IL-1beta plus IL-2. In contrast to IL-7, continuous culture in IL-1beta and IL-2 modified NK-22 cytokine profiles. IL-1beta promoted constitutive IL-22 secretion rather than acute IL-22 production in response to IL-23 and induced IL-17 in some cells. IL-2 reduced secretion of IL-22 and IL-17, increasing production of IFN-gamma and leukemia inhibitory factor. Functional deviation toward IFN-gamma production also was induced by continuous culture in IL-23. These results demonstrate the functional plasticity of NK-22 cells, which may allow flexible responses to different pathogens. Finally, we found that NK-22 cells released the B-cell survival factor, B-cell activating factor belonging to the TNF family (BAFF), suggesting a potential role of NK-22 cells in promoting B-cell-mediated mucosal immunity.

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http://linkedlifedata.com/resource/pubmed/journal/7505876

http://linkedlifedata.com/resource/pubmed/chemical/B-Cell Activating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Cytokines, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/IL2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/IL23A protein, human, http://linkedlifedata.com/resource/pubmed/chemical/IL7 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23 Subunit p19, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7, http://linkedlifedata.com/resource/pubmed/chemical/Interleukins, http://linkedlifedata.com/resource/pubmed/chemical/LIF protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Leukemia Inhibitory Factor, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/TNFSF13B protein, human, http://linkedlifedata.com/resource/pubmed/chemical/interleukin-22

Jun

pubmed-author:CellaMarinaM, pubmed-author:ColonnaMarcoM, pubmed-author:OteroKarelK

15

NLM

10961-6

pubmed-meshheading:20534450-B-Cell Activating Factor, pubmed-meshheading:20534450-Base Sequence, pubmed-meshheading:20534450-Cell Proliferation, pubmed-meshheading:20534450-Cytokines, pubmed-meshheading:20534450-DNA Primers, pubmed-meshheading:20534450-Humans, pubmed-meshheading:20534450-Interferon-gamma, pubmed-meshheading:20534450-Interleukin-1beta, pubmed-meshheading:20534450-Interleukin-2, pubmed-meshheading:20534450-Interleukin-23 Subunit p19, pubmed-meshheading:20534450-Interleukin-7, pubmed-meshheading:20534450-Interleukins, pubmed-meshheading:20534450-Killer Cells, Natural, pubmed-meshheading:20534450-Leukemia Inhibitory Factor, pubmed-meshheading:20534450-Lymphocyte Subsets, pubmed-meshheading:20534450-Palatine Tonsil, pubmed-meshheading:20534450-RNA, Messenger, pubmed-meshheading:20534450-Recombinant Proteins

Expansion of human NK-22 cells with IL-7, IL-2, and IL-1beta reveals intrinsic functional plasticity.

Journal Article, In Vitro, Research Support, N.I.H., Extramural