Linked Life Data

20534350

Source:http://linkedlifedata.com/resource/pubmed/id/20534350

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-6-10
pubmed:abstractText
We used a droplet-based microfluidic system to perform a quantitative cell-based reporter gene assay for a nuclear receptor ligand. Single Bombyx mori cells are compartmentalized in nanoliter droplets which function as microreactors with a >1000-fold smaller volume than a microtiter-plate well, together with eight or ten discrete concentrations of 20-hydroxyecdysone, generated by on-chip dilution over 3 decades and encoded by a fluorescent label. The simultaneous measurement of the expression of green fluorescent protein by the reporter gene and of the fluorescent label allows construction of the dose-response profile of the hormone at the single-cell level. Screening approximately 7500 cells per concentration provides statistically relevant data that allow precise measurement of the EC(50) (70 nM +/- 12%, alpha = 0.05), in agreement with standard methods as well as with literature data.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1879-1301
pubmed:author
pubmed:copyrightInfo
2010 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
28
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
528-36
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Quantitative cell-based reporter gene assays using droplet-based microfluidics.
pubmed:affiliation
Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, CNRS UMR 7006, 8 Allée Gaspard Monge, BP 70028, F-67083 Strasbourg Cedex, France. jc.baret@unistra.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't