Linked Life Data

20533434

Source:http://linkedlifedata.com/resource/pubmed/id/20533434

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-10-20
pubmed:abstractText
Accurate values of partition and diffusion coefficients within the lower skin layers are among the information required to estimate skin concentrations of permeants following topical application. For highly lipophilic compounds these parameters also play a significant role in determining systemic absorption rates. In this study methodology is described for making more accurate in vitro measurements of partition and diffusion coefficients in dermis. Isolated human dermis obtained from surgical reduction was mounted in side-by-side diffusion cells in the presence and absence of a dialysis membrane (5000 MW cut-off) placed between the dermis and the donor solution. Permeation of N,N-diethyltoluamide (DEET) and diclofenac across the composite membrane system, as well as steady-state skin concentrations, were measured by radiochemical techniques. For the highly protein bound compound, diclofenac, dermis permeability, and partition coefficient determined in the presence of the dialysis membrane were significantly higher than those determined in its absence. No significant differences were observed for the moderately protein bound DEET. The results show that, in the absence of a stratum corneum barrier, attention must be given to the diffusion of soluble proteins in order to obtain accurate estimates of transport and partitioning parameters for highly protein bound solutes in dermis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1520-6017
pubmed:author
pubmed:copyrightInfo
© 2010 Wiley-Liss, Inc. and the American Pharmacists Association
pubmed:issnType
Electronic
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4928-39
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Improved method for determining partition and diffusion coefficients in human dermis.
pubmed:affiliation
James L. Winkle College of Pharmacy, Academic Health Center, University of Cincinnati, Cincinnati, Ohio, USA.
pubmed:publicationType
Journal Article, Comparative Study