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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2010-11-8
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pubmed:abstractText |
In this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (HRP) on SiO?/BSA/Au/thionine/nafion-modified gold electrode was fabricated successfully. Firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (Thi) as an electron mediator via the ion-exchange interaction between the Thi and nafion. Subsequently, the SiO?/BSA/Au composite nanoparticles were assembled onto Thi film through the covalent bounding with the amino groups of Thi. Finally, HRP was immobilized on the SiO?/BSA/Au composite nanoparticles due to the covalent conjugation to construct an enzyme biosensor. The surface topographies of the SiO?/BSA/Au composite nanoparticles were investigated by using scanning electronic microscopy. The stepwise self-assemble procedure of the biosensor was further characterized by means of cyclic voltammetry and chronoamperometry. The enzyme biosensor showed high sensitivity, good stability and selectivity, a wide linear response to hydrogen peroxide (H?O?) in the range of 8.0?x?10?? ?~3.72?x?10?³ mol/L, with a detection limit of 2.0?x?10?? mol/L. The Michaelies-Menten constant K(app)(M) value was estimated to be 2.3 mM.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes, Immobilized,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorocarbon Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Gold,
http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Phenothiazines,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/perfluorosulfonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/thionine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1559-0291
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2189-96
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pubmed:meshHeading |
pubmed-meshheading:20532672-Animals,
pubmed-meshheading:20532672-Biosensing Techniques,
pubmed-meshheading:20532672-Buffers,
pubmed-meshheading:20532672-Cattle,
pubmed-meshheading:20532672-Electrochemistry,
pubmed-meshheading:20532672-Electrodes,
pubmed-meshheading:20532672-Enzymes, Immobilized,
pubmed-meshheading:20532672-Fluorocarbon Polymers,
pubmed-meshheading:20532672-Gold,
pubmed-meshheading:20532672-Horseradish Peroxidase,
pubmed-meshheading:20532672-Hydrogen Peroxide,
pubmed-meshheading:20532672-Hydrogen-Ion Concentration,
pubmed-meshheading:20532672-Nanocomposites,
pubmed-meshheading:20532672-Phenothiazines,
pubmed-meshheading:20532672-Reproducibility of Results,
pubmed-meshheading:20532672-Serum Albumin, Bovine,
pubmed-meshheading:20532672-Silicon Dioxide,
pubmed-meshheading:20532672-Surface Properties
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pubmed:year |
2010
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pubmed:articleTitle |
Enzyme biosensor based on the immobilization of HRP on SiO?/BSA/Au composite nanoparticles.
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pubmed:affiliation |
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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