Linked Life Data

20530880

Source:http://linkedlifedata.com/resource/pubmed/id/20530880

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-6-28
pubmed:abstractText
Analysis of fucosylated glycoconjugates in rat prostate by lectin blotting with Aleuria aurantia lectin revealed that the tissue contained characteristic low-molecular weight species (prostatic fucosylated glycoconjugates, PFGs). PFGs were detected almost exclusively in the ventral lobe of the prostate among rat tissues examined, and showed an apparent positive androgen dependency, that is, the content of PFGs decreased after castration and then slowly recovered upon androgen replacement. PFGs were resistant to solubilization with Triton X-100, but could be extracted with chloroform-methanol. Treatment of the crude PFG preparation with endoglycoceramidase resulted in the complete disappearance of PFGs. PFGs were also susceptible to alpha1,3/4-L-fucosidase but not to peptide-N-glycosidase F. Prostate-specific expression of PFGs was confirmed by thin layer chromatography and subsequent blot analysis. These results suggest that PFGs are fucosylated glycosphingolipid species specifically expressed in the rat ventral prostate, and that PFG expression is associated with some androgen-regulated processes in the tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1347-6947
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1261-6
pubmed:dateRevised
2010-12-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Tissue-specific expression of fucosylated glycosphingolipid species in rat prostate.
pubmed:affiliation
Division of Research Instrument and Equipment, Life Science Research Center, Institute of Research Promotion, Kagawa University, Kagawa, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't