Source:http://linkedlifedata.com/resource/pubmed/id/20528145
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-1-24
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| pubmed:abstractText |
Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX(®)) and/or poly(70L-lactide-co-30DL-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM?+?SVF, (4) DBM + PLA, and (5) DBM + PLA?+?SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM +?SVF and DBM + PLA +?SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM +?SVF: 57.69%; DBM + PLA: 24.86%; DBM + PLA +?SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM +?SVF and DBM + PLA +?SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Veratrum Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/cyclopamine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1557-8534
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
20
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
233-42
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| pubmed:meshHeading |
pubmed-meshheading:20528145-Adipose Tissue,
pubmed-meshheading:20528145-Animals,
pubmed-meshheading:20528145-Antigens, CD,
pubmed-meshheading:20528145-Antigens, Differentiation,
pubmed-meshheading:20528145-Bone Demineralization Technique,
pubmed-meshheading:20528145-Bone Density,
pubmed-meshheading:20528145-Bone Matrix,
pubmed-meshheading:20528145-Bone Regeneration,
pubmed-meshheading:20528145-Cell Differentiation,
pubmed-meshheading:20528145-Hedgehog Proteins,
pubmed-meshheading:20528145-Implants, Experimental,
pubmed-meshheading:20528145-Mice,
pubmed-meshheading:20528145-Mice, Nude,
pubmed-meshheading:20528145-Osteoblasts,
pubmed-meshheading:20528145-Random Allocation,
pubmed-meshheading:20528145-Rats,
pubmed-meshheading:20528145-Rats, Sprague-Dawley,
pubmed-meshheading:20528145-Signal Transduction,
pubmed-meshheading:20528145-Skull,
pubmed-meshheading:20528145-Stem Cell Transplantation,
pubmed-meshheading:20528145-Stromal Cells,
pubmed-meshheading:20528145-Tissue Scaffolds,
pubmed-meshheading:20528145-Veratrum Alkaloids
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| pubmed:year |
2011
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| pubmed:articleTitle |
In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model.
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| pubmed:affiliation |
Department of Plastic and Reconstructive Surgery, Inje University Ilsan Paik Hospital, Goyang City, Gyeonggi-do, Republic of Korea.
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| pubmed:publicationType |
Journal Article
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