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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1991-7-19
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pubmed:abstractText |
LAP267 is a lacI activator protein (LAP) containing an insertion of the transcriptional activation domain of the herpes simplex virus virion protein 16 within the inducer-binding and dimerization domain of the lac repressor protein. LAP267 strongly induces expression in a conditional manner from a minimal simian virus 40 early promoter linked to lac operator sequences. LAP267 is temperature-sensitive, activating expression at 32 degrees C but not at 39.5 degrees C. It is allosterically regulated in a manner opposite that of wild-type lac repressor, in that LAP267 activity is rescued at the nonpermissive temperature by isopropyl beta-D-thiogalactopyranoside (IPTG). Stable mouse cell lines containing both the LAP267 gene and a LAP-inducible chloramphenicol acetyltransferase (CAT) reporter gene were readily established and exhibited up to a 1200-fold increase in CAT activity within 24 hr upon addition of IPTG. Thus, LAP267 is a powerful inducible switch in mammalian cells, imparting a regulatory stringency similar to that observed with lac repressor in Escherichia coli.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-114666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-14337495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2157024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2162473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2247053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2426277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2440031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2546123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2664783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2830021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2830022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2830990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2843425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-286319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2985280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-2993866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3028641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3034429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3047590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3124961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3257575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3526329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3535879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-385890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-385891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-3907859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-4924012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-4932654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6096007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6264458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6278311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6292722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6300840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6369330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6462229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2052587-931989
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:geneSymbol |
lac
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5072-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2052587-Animals,
pubmed-meshheading:2052587-Chimera,
pubmed-meshheading:2052587-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2052587-DNA,
pubmed-meshheading:2052587-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2052587-Enzyme Induction,
pubmed-meshheading:2052587-HeLa Cells,
pubmed-meshheading:2052587-Humans,
pubmed-meshheading:2052587-Isopropyl Thiogalactoside,
pubmed-meshheading:2052587-Kinetics,
pubmed-meshheading:2052587-Mammals,
pubmed-meshheading:2052587-Repressor Proteins,
pubmed-meshheading:2052587-Temperature,
pubmed-meshheading:2052587-Trans-Activators,
pubmed-meshheading:2052587-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside.
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pubmed:affiliation |
Howard Hughes Medical Institute, Princeton, NJ 08544.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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