Linked Life Data

20524120

Source:http://linkedlifedata.com/resource/pubmed/id/20524120

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-7-14
pubmed:abstractText
A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6, 61.2, 42.5 and 39.8% identity with that of Arabidopsis RTH, RTE1, tomato GRL2, GRL1 and GR, respectively. Northern hybridization indicated that Rh-RTH1 expression is enhanced by endogenous and exogenous ethylene and inhibited by 1-MCP in petals and gynoecia. Rh-RTH1 expression partly correlated with sites of the ethylene receptor gene Rh-ETR1 and Rh-ETR3 expression, such as the petals, gynoecia, roots, and buds. The induction of Rh-RTH1 and Rh-ETR3 expression was substantially suppressed by 1-MCP treatment, while Rh-ETR1 expression was not reduced by 1-MCP treatment. Following treatment of flowers with sucrose, the level of Rh-RTH1 and Rh-ETR3 mRNA was only slightly decreased in petals and gynoecia. Upon wounding treatment, Rh-RTH1, Rh-ETR1 and Rh-ETR3 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The expression of Rh-RTH1 showed partial correlation with that of Rh-ETR1 and Rh-ETR3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1432-203X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
895-904
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Relationship between Rh-RTH1 and ethylene receptor gene expression in response to ethylene in cut rose.
pubmed:affiliation
College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't