Linked Life Data

20521234

Source:http://linkedlifedata.com/resource/pubmed/id/20521234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-6-3
pubmed:abstractText
Killer lymphocytes induce apoptosis by the release of cytotoxic mediators from specialized secretory lysosomes, called cytotoxic granules, into the immunological synapse formed with a cell targeted for elimination. Methods are presented here for isolating CTL and NK cell cytotoxic granules using cell disruption by nitrogen cavitation followed by continuous Percoll density gradient fractionation. Protocols are also given for purifying the key cytolytic molecules (perforin, granzyme A, granzyme B, and granulysin) from isolated cytotoxic granules by fast protein liquid chromatography.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1934-2616
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit3.37
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Isolation of cytotoxic T cell and NK granules and purification of their effector proteins.
pubmed:affiliation
Program in Cellular and Molecular Medicine, Children's Hospital and Immune Disease Institute, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural