Linked Life Data

20517599

Source:http://linkedlifedata.com/resource/pubmed/id/20517599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-7-2
pubmed:abstractText
FTIR microscopy with a focal plane array (FPA) of detectors enables routine chemical imaging on individual cells in only a few minutes. The brilliance of synchrotron radiation (SR) IR sources may enhance the signal obtained from such small biosamples containing small amounts of organic matter. We investigated individual cells obtained from a cell culture specifically developed for transmission FTIR imaging using either a Globar or an SR source coupled to the same instrumentation. SR-IR source focussing was optimized to control the energy distribution on the FPA of detectors. Here we show that accessing the IR absorption distribution from all the organic contents of cells at 1 x 1 microm pixel resolution was possible only with high circulating current (> or = 1.2 A) illuminating a limited number of the FPA's detectors to increase the signal-to-noise ratio of IR images. Finally, a high-current SR ring is mandatory for collecting FTIR images of biosamples with a high contrast in minutes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
397
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2123-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging.
pubmed:affiliation
Université de Bordeaux 2, CNRS UMR 5084, B8 Avenue des Facultés, 33405 Pessac-Cedex, France. cyril.petibois@u-bordeaux2.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't