Linked Life Data

20517556

Source:http://linkedlifedata.com/resource/pubmed/id/20517556

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-6-2
pubmed:abstractText
A new method for in-gel sample processing and tryptic digestion of proteins is described. Sample preparation, rehydration, in situ digestion and peptide extraction from gel slices are dramatically accelerated by treating the gel slice with surface acoustic waves (SAWs). Only 30 minutes total workflow time is required for this new method to produce base peak chromatograms (BPCs) of similar coverage and intensity to those observed for traditional processing and overnight digestion. Simple set up, good reproducibility, excellent peptide recoveries, rapid turnover of samples and high confidence protein identifications put this technology at the fore-front of the next generation of proteomics sample processing tools.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1473-0197
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1518-20
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Rapid microscale in-gel processing and digestion of proteins using surface acoustic waves.
pubmed:affiliation
School of Chemistry, Monash University, PO Box 23, Melbourne, 3800, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't