Linked Life Data

20515659

Source:http://linkedlifedata.com/resource/pubmed/id/20515659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-7-26
pubmed:abstractText
Hoxb3 plays important roles in embryogenesis and it has a complex transcription profile of mRNAs, non-coding RNAs and anti-sense RNAs. Characterization of the spatial expression patterns of these RNAs is important to understand their functions. We investigated the regulation and spatial expression patterns of multiple RNA transcripts derived from the Hoxb3 gene locus. By 5'-RACE we identified four novel transcription initiation sites and initiating exons, and by luciferase activity assay we identified a new promoter region. Expression pattern analysis of the alternative transcripts containing specific initiation exons in mouse embryos suggests that there are co-operations between the initiation exons, their adjacent promoters and enhancer elements to orchestrate overlapping neural tube specific transcription profiles for Hoxb3. Furthermore, we showed that anti-sense transcripts derived from the Hoxb3 locus were expressed in the hindbrain with distinct rhombomere boundaries, in a pattern complementary to the sense coding mRNA transcripts. Our results suggest that the multiple non-coding RNAs could be involved in the regulation of Hoxb3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
398
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Multiple coding and non-coding RNAs in the Hoxb3 locus and their spatial expression patterns during mouse embryogenesis.
pubmed:affiliation
Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 3/F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't