Linked Life Data

20513400

Source:http://linkedlifedata.com/resource/pubmed/id/20513400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-6-1
pubmed:abstractText
Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior incubation at low temperatures. This property is indicative of the ability of these peptides to cluster CL away from DMPG. In contrast, the well studied antimicrobial cationic peptide magainin 2 does not cluster anionic lipids. We ascribe the lower anionic lipid clustering ability of magainin to its low density of positive charges compared with the five other AHCAPs used in this work. The peptide MSI-1254 was particularly potent in segregating these two anionic lipids. Consequently, clusters enriched in DMPG appear in a lipid mixture with CL. These can rapidly form higher temperature crystalline phases because of the increased permeability of the bilayer caused by the AHCAPs. The polyaminoacids, poly-L-Lysine and poly-l-arginine are also very effective in causing this segregation. Thus, the clustering of anionic lipids by AHCAPs is not confined only to mixtures of anionic with zwitterionic lipids, but it extends to mixtures containing different anionic headgroups. The resulting effects, however, have different consequences to the biological activity. This finding broadens the scope for which an AHCAP agent will cluster lipids in a membrane.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-10663530, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-10931439, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-11259300, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-1420881, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-17599802, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-17983614, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-18498774, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-18500827, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-18822270, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-18826221, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19118516, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19383470, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19427300, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19576192, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19581460, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-19843179, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-2546595, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-3954999, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-3955044, http://linkedlifedata.com/resource/pubmed/commentcorrection/20513400-9017203
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1542-0086
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2564-73
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Amphipathic helical cationic antimicrobial peptides promote rapid formation of crystalline states in the presence of phosphatidylglycerol: lipid clustering in anionic membranes.
pubmed:affiliation
Department of Biochemistry and Biomedical Sciences, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural