Linked Life Data

20509218

Source:http://linkedlifedata.com/resource/pubmed/id/20509218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-5-28
pubmed:abstractText
l. In the hepatopancreas of the shrimp Penaeus japonicus the beta-glucosidase is present, either free or membrane-bound. The specific activity of the purified enzyme is 237,333 units/mg of protein and 191,111 units/mg of protein for the free and the membrane-bound beta-glucosidase, respectively. 2.The non-membrane-bound beta-glucosidase appears to be the same molecular size as the membrane-bound enzyme, both being monomers and consisting of a polypeptide chain of apparent M(r) 65,000, as estimated by chromatography on Superose 12 and by SDS-PAGE. 3.Both enzymes share similarities in their molecular size and substrate specificities (with ratios of 100:17:4:12 for the non-membrane-bound and 100:32:13:12 for the membrane-bound enzyme for the activities with methylumbelliferyl-beta-D-glucoside, methylumbelliferyl-beta-D-galactoside, methylumbelliferyl-alpha -L-arabinopyranoside and methylumbelliferyl-beta-xyloside respectively, as substrates). 4.The membrane-bound beta-glucosidase can be differentiated from the non-membrane-bound enzyme by its isoelectric point (7.5 vs 6.6), Km (182 micrometer vs 76 micrometer), pH optimum (4.5 vs 5.5), phosphorylation,sialyation and thermostability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-0491
pubmed:author
pubmed:issnType
Print
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-83
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Comparative study of free and membrane-bound acidic beta-D-glucosidase from the hepatopancreas of the shrimp Penaeus japonicus (Crustacea: decapoda).
pubmed:affiliation
Division of Biochemistry and Molecular Science, Institute of Zoology, Academia Sinica, Nankang, Taipei,Republic of China.
pubmed:publicationType
Journal Article, Comparative Study