Linked Life Data

20507709

Source:http://linkedlifedata.com/resource/pubmed/id/20507709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8-9
pubmed:dateCreated
2010-5-28
pubmed:abstractText
Solid free-form fabrication (SFF) technology is used to fabricate scaffolds with controllable characteristics including well-defined pore size and porosity. The multi-head deposition system (MHDS), one form of SFF technology, may be more advantageous than others for fabricating scaffolds because a MHDS does not require the use of a cytotoxic solvent. This method, however, may induce the thermal degradation of raw materials and a subsequent decrease in the material's molecular weight, whereby hydrolytic degradation, resulting in acidic by-products, might be accelerated. This study investigated whether fabrication of poly(lactic-co-glycolic acid) (PLGA) scaffolds using a MHDS with various residence times in the heating step induces thermal degradation and affects the proliferation of cells seeded on the scaffold in vitro. To answer this question, we fabricated porous three-dimensional PLGA scaffolds using residence times of 1, 3, 5 and 7 days for groups 1 through 4, respectively. Degradation behavior of the scaffolds was observed for 7 weeks in phosphate-buffered saline solution (pH 7.4) at 37 degrees C. The molecular weight, glass transition temperature and mechanical properties were compared for PLGA scaffolds fabricated with each of the four residence times at 120 degrees C. The proliferation rate of MC3T3-E1 cells grown on each group of scaffolds was compared to investigate the effect of acidic by-products on the growth of seeded cells in vitro. The heat process applied in fabrication of SFF-based PLGA scaffolds induced considerable thermal degradation followed by a decrease in molecular weight and mechanical compressive strength of the scaffolds in groups 3 and 4, which had more than 3 days residence time. Moreover, the cell proliferation rate was significantly higher for group 1 than for groups 3 and 4.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1568-5624
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-80
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Effect of thermal degradation of SFF-based PLGA scaffolds fabricated using a multi-head deposition system followed by change of cell growth rate.
pubmed:affiliation
Department of Mechanical Engineering, POSTECH, Gyungbuk, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't