|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0162610,
umls-concept:C0205245,
umls-concept:C0206588,
umls-concept:C0242290,
umls-concept:C1514562,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2010-8-23
|
|
pubmed:abstractText |
NR4A nuclear receptors are a diverse group of orphan nuclear receptors with critical roles in regulating cell proliferation and cell differentiation. The ortholog of the NR4A nuclear receptor in Caenorhabditis elegans, NHR-6, also has a role in cell proliferation and cell differentiation during organogenesis of the spermatheca. Here we show that NHR-6 is able to bind the canonical NR4A monomer response element and can transactivate from this site in mammalian HEK293 cells. Using a functional GFP-tagged NHR-6 fusion, we also demonstrate that NHR-6 is nuclear localized during development of the spermatheca. Mutation of the DNA-binding domain of NHR-6 abolishes its activity in genetic rescue assays, demonstrating a requirement for the DNA-binding domain. This study represents the first genetic demonstration of an in vivo requirement for an NR4A nuclear receptor DNA-binding domain in a whole organism.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-10601324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-10947977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-11553630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-12381721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-12709428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-12774125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-12809604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-1314418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-14612408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-15871945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-16604165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-17418373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-17515897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-17981763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-18096150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-1925541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-19515692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-3191912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-4366476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-7705655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-7758108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20506374-9604929
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1526-968X
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
(c) 2010 Wiley-Liss, Inc.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
48
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
485-91
|
|
pubmed:dateRevised |
2011-8-3
|
|
pubmed:meshHeading |
pubmed-meshheading:20506374-Animals,
pubmed-meshheading:20506374-Animals, Genetically Modified,
pubmed-meshheading:20506374-Binding Sites,
pubmed-meshheading:20506374-Caenorhabditis elegans,
pubmed-meshheading:20506374-Caenorhabditis elegans Proteins,
pubmed-meshheading:20506374-Cell Nucleus,
pubmed-meshheading:20506374-DNA,
pubmed-meshheading:20506374-Genetic Complementation Test,
pubmed-meshheading:20506374-Gonads,
pubmed-meshheading:20506374-Green Fluorescent Proteins,
pubmed-meshheading:20506374-HEK293 Cells,
pubmed-meshheading:20506374-Humans,
pubmed-meshheading:20506374-Microscopy, Fluorescence,
pubmed-meshheading:20506374-Mutation,
pubmed-meshheading:20506374-Organogenesis,
pubmed-meshheading:20506374-Protein Binding,
pubmed-meshheading:20506374-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:20506374-Response Elements,
pubmed-meshheading:20506374-Transfection
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
A functional NR4A nuclear receptor DNA-binding domain is required for organ development in Caenorhabditis elegans.
|
|
pubmed:publicationType |
Letter,
Research Support, N.I.H., Extramural
|
