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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2010-7-20
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pubmed:abstractText |
Tat is a multifunctional transactivator encoded by human immunodeficiency virus type 1 (HIV-1). Tat transactivating activity is controlled by nicotinamide adenine nucleotide(+) (NAD(+))-dependent deacetylase sirtuin 1 (SIRT1). Nicotinamide phosphoribosyltransferase (Nampt) is a rate-limiting enzyme in the conversion of nicotinamide into NAD(+), which is crucial for SIRT1 activation. Thus, the effect of Nampt on Tat-regulated SIRT activity was studied in Hela-CD4-beta-gal (MAGI) cells. We demonstrated that Tat caused NAD(+) depletion and inhibited Nampt mRNA and protein expression in MAGI cells. Resveratrol reversed Tat-induced NAD(+) depletion and inhibition of Nampt mRNA and protein expression. Further investigation revealed that Tat-induced inhibition of SIRT1 activity was potentiated in Nampt-knockdown by Nampt siRNA compared to treatment with Tat alone. Nampt siRNA potentiated Tat-induced HIV-1 transactivation in MAGI cells. Altogether, these results indicate that Nampt is critical in the regulation of Tat-induced inhibition of SIRT1 activity and long terminal repeat (LTR) transactivation. Nampt/SIRT1 pathway could be a novel therapeutic tool for the treatment of HIV-1 infection.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/NAD,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinamide...,
http://linkedlifedata.com/resource/pubmed/chemical/Sirtuin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Stilbenes,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/resveratrol,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1097-4644
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2010 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
110
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1464-70
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pubmed:meshHeading |
pubmed-meshheading:20506278-Antigens, CD4,
pubmed-meshheading:20506278-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:20506278-Dose-Response Relationship, Drug,
pubmed-meshheading:20506278-HIV Long Terminal Repeat,
pubmed-meshheading:20506278-HeLa Cells,
pubmed-meshheading:20506278-Humans,
pubmed-meshheading:20506278-Immunoblotting,
pubmed-meshheading:20506278-NAD,
pubmed-meshheading:20506278-Nicotinamide Phosphoribosyltransferase,
pubmed-meshheading:20506278-RNA Interference,
pubmed-meshheading:20506278-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20506278-Signal Transduction,
pubmed-meshheading:20506278-Sirtuin 1,
pubmed-meshheading:20506278-Stilbenes,
pubmed-meshheading:20506278-Transcriptional Activation,
pubmed-meshheading:20506278-beta-Galactosidase,
pubmed-meshheading:20506278-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2010
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pubmed:articleTitle |
Nicotinamide phosphoribosyltransferase/sirtuin 1 pathway is involved in human immunodeficiency virus type 1 Tat-mediated long terminal repeat transactivation.
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pubmed:affiliation |
College of Life Science & Bioengineering, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing 100124, China. zhanghs@bjut.edu.cn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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