Linked Life Data

20506212

Source:http://linkedlifedata.com/resource/pubmed/id/20506212

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-7-23
pubmed:abstractText
Despite the extensive interest in applications of plasmid DNA, there have been few direct measurements of the root mean square radius of gyration, R(G), of different plasmid isoforms over a broad range of plasmid size. Static light scattering data were obtained using supercoiled, open-circular, and linear isoforms of 5.76, 9.80, and 16.8 kbp plasmids. The results from this study extend the range of R(G) values available in the literature to plasmid sizes typically used for gene therapy and DNA vaccines. The experimental data were compared with available theoretical expressions based on the worm-like chain model, with the best-fit value of the apparent persistence length for both the linear and open-circular isoforms being statistically identical at 46 nm. A new expression was developed for the radius of gyration of the supercoiled plasmid based on a model for linear DNA using an effective contour length that is equal to a fraction of the total contour length. These results should facilitate the development of micro/nano-fluidic devices for DNA manipulation and size-based separation processes for plasmid DNA purification.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1097-0290
pubmed:author
pubmed:copyrightInfo
2010 Wiley Periodicals, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-42
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Radius of gyration of plasmid DNA isoforms from static light scattering.
pubmed:affiliation
Department of Chemical Engineering, The Pennsylvania State University, University Park, 16802, USA.
pubmed:publicationType
Journal Article, Comparative Study