| pubmed-article:20504287 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20504287 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:20504287 | lifeskim:mentions | umls-concept:C0038250 | lld:lifeskim |
| pubmed-article:20504287 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:20504287 | lifeskim:mentions | umls-concept:C0521457 | lld:lifeskim |
| pubmed-article:20504287 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:20504287 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:20504287 | pubmed:dateCreated | 2010-5-27 | lld:pubmed |
| pubmed-article:20504287 | pubmed:abstractText | INTRODUCTION : The ability to expand organ-specific stem/progenitor cells is critical for translational applications, although uncertainties often arise in identifying the lineage of expanded cells. Therefore, superior insights into lineage maintenance mechanisms will be helpful for cell/gene therapy. METHODS : We studied epithelial cells isolated from fetal human pancreas to assess their proliferation potential, changes in lineage markers during culture, and capacity for generating insulin-expressing beta cells. Cells were isolated by immunomagnetic sorting for epithelial cell adhesion molecule (EpCAM), and characterized for islet-associated transcription factors, hormones, and ductal markers. Further studies were performed after modification of cells with the catalytic subunit of human telomerase reverse transcriptase (hTERT). RESULTS : Fetal pancreatic progenitor cells efficiently formed primary cultures, although their replication capacity was limited. This was overcome by introduction and expression of hTERT with a retroviral vector, which greatly enhanced cellular replication in vitro. However, we found that during culture hTERT-modified pancreatic progenitor cells switched their phenotype with gain of additional mesodermal properties. This phenotypic switching was inhibited when a pancreas-duodenal homeobox (Pdx)-1 transgene was expressed in hTERT-modified cells with a lentiviral vector, along with inductive signaling through activin A and serum deprivation. This restored endocrine properties of hTERT-modified cells in vitro. Moreover, transplantation studies in immunodeficient mice verified the capacity of these cells for expressing insulin in vivo. CONCLUSIONS : Limited replication capacity of pancreatic endocrine progenitor cells was overcome by the hTERT mechanism, which should facilitate further studies of such cells, although mechanisms regulating switches between meso-endodermal fates of expanded cells will need to be controlled for developing specific applications. The availability of hTERT-expanded fetal pancreatic endocrine progenitor cells will be helpful for studying and recapitulating stage-specific beta lineage advancement in pluripotent stem cells. | lld:pubmed |
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| pubmed-article:20504287 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20504287 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20504287 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:20504287 | pubmed:issn | 1757-6512 | lld:pubmed |
| pubmed-article:20504287 | pubmed:author | pubmed-author:ChengKangK | lld:pubmed |
| pubmed-article:20504287 | pubmed:author | pubmed-author:GuptaSanjeevS | lld:pubmed |
| pubmed-article:20504287 | pubmed:author | pubmed-author:FollenziAnton... | lld:pubmed |
| pubmed-article:20504287 | pubmed:author | pubmed-author:FleischerNorm... | lld:pubmed |
| pubmed-article:20504287 | pubmed:author | pubmed-author:SuranaManjuM | lld:pubmed |
| pubmed-article:20504287 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20504287 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:20504287 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20504287 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20504287 | pubmed:pagination | 6 | lld:pubmed |
| pubmed-article:20504287 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20504287 | pubmed:articleTitle | Switching of mesodermal and endodermal properties in hTERT-modified and expanded fetal human pancreatic progenitor cells. | lld:pubmed |
| pubmed-article:20504287 | pubmed:affiliation | Hepatology Division, Department of Medicine, Albert Einstein College of Medicine, Ullmann Bldg, Rm 625, 1300 Morris Park Avenue, Bronx, NY 10461, USA. kcheng@aecom.yu.edu. | lld:pubmed |
| pubmed-article:20504287 | pubmed:publicationType | Journal Article | lld:pubmed |
