Source:http://linkedlifedata.com/resource/pubmed/id/20504287
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2010-5-27
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| pubmed:abstractText |
INTRODUCTION : The ability to expand organ-specific stem/progenitor cells is critical for translational applications, although uncertainties often arise in identifying the lineage of expanded cells. Therefore, superior insights into lineage maintenance mechanisms will be helpful for cell/gene therapy. METHODS : We studied epithelial cells isolated from fetal human pancreas to assess their proliferation potential, changes in lineage markers during culture, and capacity for generating insulin-expressing beta cells. Cells were isolated by immunomagnetic sorting for epithelial cell adhesion molecule (EpCAM), and characterized for islet-associated transcription factors, hormones, and ductal markers. Further studies were performed after modification of cells with the catalytic subunit of human telomerase reverse transcriptase (hTERT). RESULTS : Fetal pancreatic progenitor cells efficiently formed primary cultures, although their replication capacity was limited. This was overcome by introduction and expression of hTERT with a retroviral vector, which greatly enhanced cellular replication in vitro. However, we found that during culture hTERT-modified pancreatic progenitor cells switched their phenotype with gain of additional mesodermal properties. This phenotypic switching was inhibited when a pancreas-duodenal homeobox (Pdx)-1 transgene was expressed in hTERT-modified cells with a lentiviral vector, along with inductive signaling through activin A and serum deprivation. This restored endocrine properties of hTERT-modified cells in vitro. Moreover, transplantation studies in immunodeficient mice verified the capacity of these cells for expressing insulin in vivo. CONCLUSIONS : Limited replication capacity of pancreatic endocrine progenitor cells was overcome by the hTERT mechanism, which should facilitate further studies of such cells, although mechanisms regulating switches between meso-endodermal fates of expanded cells will need to be controlled for developing specific applications. The availability of hTERT-expanded fetal pancreatic endocrine progenitor cells will be helpful for studying and recapitulating stage-specific beta lineage advancement in pluripotent stem cells.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-10700229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-10835641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-10847584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-10856888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-12021244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-12403815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-12557149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-12756298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-15677631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-15918158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-16123344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-16491084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-16780202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18157115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18238854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18243096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18319302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18398419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-18754011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-19287398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-19669279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-9040956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-9726230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20504287-9916802
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:issn |
1757-6512
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
1
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6
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| pubmed:year |
2010
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| pubmed:articleTitle |
Switching of mesodermal and endodermal properties in hTERT-modified and expanded fetal human pancreatic progenitor cells.
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| pubmed:affiliation |
Hepatology Division, Department of Medicine, Albert Einstein College of Medicine, Ullmann Bldg, Rm 625, 1300 Morris Park Avenue, Bronx, NY 10461, USA. kcheng@aecom.yu.edu.
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| pubmed:publicationType |
Journal Article
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