Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-7-22
pubmed:abstractText
In vivo interactions of vitamin E with diethylmaleate (DEM) and bromotrichloromethane (CBrCl3) were examined in rats fed a diet either without vitamin E or supplemented with 30 IU dl-alpha-tocopheryl acetate/kg. Groups of rats within each dietary group were given two injections 30 min apart. One group received two injections of the mineral oil carrier. The other groups were injected with either DEM and mineral oil, mineral oil and CBrCl3, or DEM and CBrCl3. The rats were killed 10 min after the second injection. Measurements were made of hepatic GSH, thiobarbituric acid-reactive substances (TBARS) as a lipid peroxidation index, and 11 enzymes as potential markers of oxidant damage. Special focus was placed on reactive cysteine-containing aldehyde dehydrogenase (ALDH). Although dietary vitamin E protected ALDH, the enzyme was highly susceptible to oxidant damage. ALDH activity was correlated with GSH (r = 0.83, p less than 0.001) and there was an inverse relationship between the logarithmic values of ALDH activity and TBARS (r = 0.78, p less than 0.001). Similar results were observed for a number of other enzymes when GSH depletion preceded oxidant treatment. Two-way analysis of variance revealed significant effects of vitamin E and of injection treatments on hepatic GSH. There was a significant interaction between vitamin E and the injection treatments on the activities of five enzymes. The results suggested that vitamin E and GSH functioned together to protect sensitive enzymes against oxidant stress. The sensitive enzymes may be useful markers of hepatic damage in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/Bromotrichloromethane, http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione, http://linkedlifedata.com/resource/pubmed/chemical/Maleates, http://linkedlifedata.com/resource/pubmed/chemical/Sulfhydryl Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Thiobarbiturates, http://linkedlifedata.com/resource/pubmed/chemical/Tocopherols, http://linkedlifedata.com/resource/pubmed/chemical/Vitamin E, http://linkedlifedata.com/resource/pubmed/chemical/alpha-Tocopherol, http://linkedlifedata.com/resource/pubmed/chemical/diethyl maleate, http://linkedlifedata.com/resource/pubmed/chemical/thiobarbituric acid
pubmed:status
MEDLINE
pubmed:issn
0891-5849
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-60
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2050297-Aldehyde Dehydrogenase, pubmed-meshheading:2050297-Animals, pubmed-meshheading:2050297-Biological Markers, pubmed-meshheading:2050297-Bromotrichloromethane, pubmed-meshheading:2050297-Eating, pubmed-meshheading:2050297-Free Radicals, pubmed-meshheading:2050297-Glutathione, pubmed-meshheading:2050297-Kinetics, pubmed-meshheading:2050297-Lipid Peroxidation, pubmed-meshheading:2050297-Maleates, pubmed-meshheading:2050297-Mitochondria, Liver, pubmed-meshheading:2050297-Rats, pubmed-meshheading:2050297-Sensitivity and Specificity, pubmed-meshheading:2050297-Substrate Specificity, pubmed-meshheading:2050297-Sulfhydryl Compounds, pubmed-meshheading:2050297-Thiobarbiturates, pubmed-meshheading:2050297-Tocopherols, pubmed-meshheading:2050297-Vitamin E, pubmed-meshheading:2050297-alpha-Tocopherol
pubmed:year
1991
pubmed:articleTitle
Vitamin E, diethylmaleate and bromotrichloromethane interactions in oxidative damage in vivo.
pubmed:affiliation
Department of Food Science and Technology, University of California, Davis 95616.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.