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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0079904,
umls-concept:C0337611,
umls-concept:C0429403,
umls-concept:C1417708,
umls-concept:C1420556,
umls-concept:C1423613,
umls-concept:C1424530,
umls-concept:C1539081,
umls-concept:C1705525,
umls-concept:C1711351
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pubmed:issue |
7
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pubmed:dateCreated |
1991-7-24
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pubmed:abstractText |
We recently reported that the apparently non-DNA-binding 65 kd subunit (p65) of the NF-kappa B transcription factor can modulate the DNA-binding specificity of the 50 kd subunit (p50) of NF-kappa B. In this study we provide an explanation for this property of p65. In electrophoretic mobility shift assays and upon UV cross-linking to DNA, gel-purified p65 is shown to be a kappa B-specific DNA-binding protein on its own. The binding activity was only detectable if high amounts of p65 were used for the analyses and after the application of a modified renaturation protocol. DNA-binding of the p65 dimer, in contrast to that of p50, was inhibited by I kappa B-alpha and -beta. This finding is consistent with a receptor function of p65 for both inhibitory subunits. Direct UV cross-linking of NF-kappa B to DNA probes which were photoreactive within only one half-site and a binding competition analysis with p65 showed that p65 has a strong preference for binding to the less conserved half site of kappa B motifs whereas p50 has a moderate preference for the more highly conserved half site. In electrophoretic mobility shift assays and upon sedimentation through glycerol gradients, NF-kappa B appears to exist as a heterodimer composed of one p50 and one p65 subunit whereas data from gel filtration suggest a higher order complex.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-1985897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2006423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2106065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2125960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2157987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2174540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2181276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2184941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2203531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2203532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2225078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2234062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2494701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2494702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2495183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2663471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-2691328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-3133660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-3140380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2050119-3678204
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1817-25
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2050119-Base Sequence,
pubmed-meshheading:2050119-Binding, Competitive,
pubmed-meshheading:2050119-Centrifugation, Density Gradient,
pubmed-meshheading:2050119-Cross-Linking Reagents,
pubmed-meshheading:2050119-Cytosol,
pubmed-meshheading:2050119-DNA Probes,
pubmed-meshheading:2050119-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2050119-Glycerol,
pubmed-meshheading:2050119-Humans,
pubmed-meshheading:2050119-Molecular Sequence Data,
pubmed-meshheading:2050119-NF-kappa B,
pubmed-meshheading:2050119-Pregnancy Proteins,
pubmed-meshheading:2050119-Structure-Activity Relationship,
pubmed-meshheading:2050119-Ultraviolet Rays
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pubmed:year |
1991
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pubmed:articleTitle |
NF-kappa B contacts DNA by a heterodimer of the p50 and p65 subunit.
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pubmed:affiliation |
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Martinsried, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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