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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 14
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pubmed:dateCreated |
2010-7-16
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pubmed:abstractText |
It has been shown that the voltage (V(m)) dependence of ClC Cl(-) channels is conferred by interaction of the protopore gate with H(+) ions. However, in this paper we present evidence which indicates that permeant Cl(-) ions contribute to V(m)-dependent gating of the broadly distributed ClC-2 Cl() channel. The apparent open probability (P(A)) of ClC-2 was enhanced either by changing the [Cl(-)](i) from 10 to 200 mM or by keeping the [Cl(-)](i) low (10 mM) and then raising [Cl(-)](o) from 10 to 140 mM. Additionally, these changes in [Cl(-)] slowed down channel closing at positive V(m) suggesting that high [Cl(-)] increased pore occupancy thus hindering closing of the protopore gate. The identity of the permeant anion was also important since the P(A)(V(m)) curves were nearly identical with Cl(-) or Br(-) but shifted to negative voltages in the presence of SCN() ions. In addition, gating, closing rate and reversal potential displayed anomalous mole fraction behaviour in a SCN(-)/Cl() mixture in agreement with the idea that pore occupancy by different permeant anions modifies the V(m) dependence ClC-2 gating. Based on the ec1-ClC anion pathway, we hypothesized that opening of the protopore gate is facilitated when Cl(-) ions dwell in the central binding site. In contrast, when Cl(-) ions dwell in the external binding site they prevent the gate from closing. Finally, this Cl(-)-dependent gating in ClC-2 channels is of physiological relevance since an increase in [Cl(-)](o) enhances channel opening when the [Cl(-)](i) is in the physiological range.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20498235-10050002,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20498235-9565403
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1469-7793
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
588
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2545-56
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Permeant anions contribute to voltage dependence of ClC-2 chloride channel by interacting with the protopore gate.
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pubmed:affiliation |
Instituto de Física, Universidad Autónoma de San Luis Potosí, Av. Dr Manuel Nava no. 6, Zona Universitaria, San Luis Potosí, SLP 78290, México.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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