Source:http://linkedlifedata.com/resource/pubmed/id/20496086
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
2010-8-3
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pubmed:abstractText |
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Intergenic,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxanthine...,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1432-8798
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1337-41
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pubmed:meshHeading |
pubmed-meshheading:20496086-Animals,
pubmed-meshheading:20496086-Capripoxvirus,
pubmed-meshheading:20496086-Cell Line,
pubmed-meshheading:20496086-DNA, Intergenic,
pubmed-meshheading:20496086-Green Fluorescent Proteins,
pubmed-meshheading:20496086-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:20496086-Luminescent Proteins,
pubmed-meshheading:20496086-Male,
pubmed-meshheading:20496086-Promoter Regions, Genetic,
pubmed-meshheading:20496086-Recombination, Genetic,
pubmed-meshheading:20496086-Testis,
pubmed-meshheading:20496086-Transfection,
pubmed-meshheading:20496086-Virus Replication
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pubmed:year |
2010
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pubmed:articleTitle |
Identification of an intergenic region that is not essential for replication of goatpox virus.
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pubmed:affiliation |
Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People's Republic of China. mawenge2004@yahoo.com.cn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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