Linked Life Data

2049340

Source:http://linkedlifedata.com/resource/pubmed/id/2049340

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-7-23
pubmed:abstractText
The human IL-3 gene, located on chromosome 5, contains several cis-acting DNA sequences, i.e. CLE (conserved lymphokine element) and a GC-rich region, similar to the GM-CSF gene. To investigate the role of these elements, the 5' flanking region of the IL-3 gene was attached to a bacterial chloramphenicol acetyltransferase (CAT) gene. The fusion plasmids were analyzed by an in vitro transcription system using Jurkat cell nuclear extract prepared from cells stimulated with phorbol-12-myristate-13-acetate and calcium ionophore (PMA/A23187), introduced into Jurkat cells, expressed transiently, and stimulated by co-transfection of human T cell leukemia virus type I (HTLV-I) encoded transactivator, p40tax. The GC-rich region enhanced TATA-dependent transcription in the in vitro transcription system and also strongly responded to p40tax stimulation in the in vivo cotransfection assay. Using this GC-rich region as a probe, we identified a constitutive DNA-protein complex, alpha, whose binding specificity correlates with transcription activity. However, this element is not sufficient for the expression of the IL-3 gene in response to T cell activation signals (PMA/A23187) and no sequence was found within the IL-3 gene which mediates the response to PMA/A23187. The enhancer sequence which responds to T cell activation signals may be located outside the IL-3 gene and may be shared by other lymphokines, possibly by GM-CSF. We propose that the GM-CSF enhancer (CLE2/GC box) which mediates the response to T cell activation signals may stimulate the expression of the IL-3 gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-54
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2049340-Base Composition, pubmed-meshheading:2049340-Base Sequence, pubmed-meshheading:2049340-Calcimycin, pubmed-meshheading:2049340-Enhancer Elements, Genetic, pubmed-meshheading:2049340-Gene Expression Regulation, pubmed-meshheading:2049340-Gene Products, tax, pubmed-meshheading:2049340-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2049340-Humans, pubmed-meshheading:2049340-Interleukin-3, pubmed-meshheading:2049340-Lymphocyte Activation, pubmed-meshheading:2049340-Molecular Sequence Data, pubmed-meshheading:2049340-Promoter Regions, Genetic, pubmed-meshheading:2049340-Sequence Homology, Nucleic Acid, pubmed-meshheading:2049340-T-Lymphocytes, pubmed-meshheading:2049340-Tetradecanoylphorbol Acetate, pubmed-meshheading:2049340-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
Definition of a GC-rich motif as regulatory sequence of the human IL-3 gene: coordinate regulation of the IL-3 gene by CLE2/GC box of the GM-CSF gene in T cell activation.
pubmed:affiliation
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't