Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1978-5-8
pubmed:abstractText
Translation in vitro of the mRNA coding for the vesicular stomatitis virus membrane glycoprotein G in a membrane-free ribosomal extract from HeLa cells allowed the synthesis of only the unglycosylated protein G1 (molecular weight, 63,000). Addition of stripped crude microsomal membranes from HeLa cells resulted in the conversion of G1 to the glycosylated protein G2 (molecular weight, 67,000). The G2 protein synthesized by the reconstructed microsomal membrane/ribosome system was found to be segregated inside the microsomal membrane vesicles and was thus protected from the proteolytic action of trypsin and chymotrypsin. Stripped membranes were required at an early stage of protein synthesis for the synthesized protein to be inserted into the membrane vesicles and to be glycosilated. The segregated protein G2, however, was not completely protected from proteolytic digestion, showing that a portion of the polypeptide chain of about 3000 daltons was present on the cytoplasmic side of the membrane vesicle. Our data thus suggest that, unlike the secretory proteins, the membrane glycoproteins are not completely discharged across the microsomal membranes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-1012329, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-1061088, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-163768, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-169263, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-186640, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-191640, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-193104, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-402922, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-4354945, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-4682341, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-5260940, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-811671, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-811672, http://linkedlifedata.com/resource/pubmed/commentcorrection/204929-837448
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
715-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
In vitro synthesis of vesicular stomatitis virus membrane glycoprotein and insertion into membranes.
pubmed:publicationType
Journal Article, In Vitro