Linked Life Data

20491465

Source:http://linkedlifedata.com/resource/pubmed/id/20491465

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-6-10
pubmed:abstractText
An assay triggered by recognition-induced charge switching is developed for protein detection and quantification. Aptamer-functionalized silica nanoparticles (NPs) have been synthesized to capture lysozyme, resulting in an alternation of the surface charge from negative to partially positive. The binding event is then translated and monitored by the fluorescence signal of a highly fluorescent anionic poly(fluorene-alt-vinylene) (PFVSO(3)), which "stains" on protein/aptamer-NP complexes via electrostatic interaction. Blue-greenish fluorescence of PFVSO(3) is observed in the presence of lysozyme by the naked eye, while no fluorescence is obtained for NPs upon treatment with a mixture of foreign proteins. A linear relationship between NP fluorescence and lysozyme is observed in the concentration range of 0-22.5 microg/mL, which gives a limit of detection as approximately 0.36 microg/mL. This work demonstrates a convenient label-free conjugated polyelectrolyte (CPE)-based protein detection with high specificity and sensitivity, which has potential applications in medical diagnosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1520-5827
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10025-30
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Anionic conjugated polymer with aptamer-functionalized silica nanoparticle for label-free naked-eye detection of lysozyme in protein mixtures.
pubmed:affiliation
Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, 117576 Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't