Source:http://linkedlifedata.com/resource/pubmed/id/20489054
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2010-8-27
|
| pubmed:abstractText |
Transforming growth factor-beta1 (TGF-beta1) is a pleiotropic cytokine with major in vitro effects on hematopoietic stem cells (HSCs) and lymphocyte development. Little is known about hematopoiesis from mice with constitutive TGF-beta1 inactivation largely because of important embryonic lethality and development of a lethal inflammatory disorder in TGF-beta1(-/-) pups, making these studies difficult. Here, we show that no sign of the inflammatory disorder was detectable in 8- to 10-day-old TGF-beta1(-/-) neonates as judged by both the number of T-activated and T-regulator cells in secondary lymphoid organs and the level of inflammatory cytokines in sera. After T-cell depletion, the inflammatory disease was not transplantable in recipient mice. Bone marrow cells from 8- to 10-day-old TGF-beta1(-/-) neonates showed strikingly impaired short- and long-term reconstitutive activity associated with a parallel decreased in vivo homing capacity of lineage negative (Lin(-)) cells. In addition an in vitro-reduced survival of immature progenitors (Lin(-) Kit(+) Sca(+)) was observed. Similar defects were found in liver cells from TGF-beta1(-/-) embryos on day 14 after vaginal plug. These data indicate that TGF-beta1 is a critical regulator for in vivo homeostasis of the HSCs, especially for their homing potential.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1528-0020
|
| pubmed:author |
pubmed-author:Bennaceur-GriscelliAnneliseA,
pubmed-author:CapronClaudeC,
pubmed-author:ChagraouiHédiaH,
pubmed-author:CharrierSabineS,
pubmed-author:Cramer-BordéElisabethE,
pubmed-author:GalyAnneA,
pubmed-author:JalbertValérieV,
pubmed-author:LécluseYannY,
pubmed-author:LacoutCatherineC,
pubmed-author:VainchenkerWilliamW
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
26
|
| pubmed:volume |
116
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1244-53
|
| pubmed:dateRevised |
2010-10-22
|
| pubmed:meshHeading |
pubmed-meshheading:20489054-Animals,
pubmed-meshheading:20489054-Animals, Newborn,
pubmed-meshheading:20489054-Autoimmune Diseases,
pubmed-meshheading:20489054-Blotting, Western,
pubmed-meshheading:20489054-Bone Marrow Cells,
pubmed-meshheading:20489054-Cell Lineage,
pubmed-meshheading:20489054-Cell Separation,
pubmed-meshheading:20489054-Cells, Cultured,
pubmed-meshheading:20489054-Cytokines,
pubmed-meshheading:20489054-Embryo, Mammalian,
pubmed-meshheading:20489054-Female,
pubmed-meshheading:20489054-Fetus,
pubmed-meshheading:20489054-Flow Cytometry,
pubmed-meshheading:20489054-Hematopoiesis,
pubmed-meshheading:20489054-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:20489054-Hematopoietic Stem Cells,
pubmed-meshheading:20489054-Inflammation,
pubmed-meshheading:20489054-Male,
pubmed-meshheading:20489054-Mice,
pubmed-meshheading:20489054-Mice, Knockout,
pubmed-meshheading:20489054-RNA, Messenger,
pubmed-meshheading:20489054-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20489054-Transforming Growth Factor beta1
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
A major role of TGF-beta1 in the homing capacities of murine hematopoietic stem cell/progenitors.
|
| pubmed:affiliation |
Inserm U1009, Faculté de Médecine Paris XI, Institut Gustave Roussy, Villejuif, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|