Source:http://linkedlifedata.com/resource/pubmed/id/20488535
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
24
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| pubmed:dateCreated |
2010-6-11
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| pubmed:abstractText |
Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. POCfs were created by reacting citric acid with 1,8-octanediol and seeded on opposing faces with human MSCs and urothelial cells; normal bladder smooth muscle cells and UCs, or unseeded POCfs. Partial cystectomized nude rats were augmented with the aforementioned POCfs, enveloped with omentum and sacrificed at 4 and 10 weeks. Isolated bladders were subjected to Trichrome and anti-human gamma-tubulin, calponin, caldesmon, smooth muscle gamma-actin, and elastin stainings. Mechanical testing of POCfs revealed a Young's modulus of 138 kPa with elongation 137% its initial length without permanent deformation demonstrating its high uniaxial elastic potential. Trichrome and immunofluorescent staining of MSC/UC POCf augmented bladders exhibited typical bladder architecture with muscle bundle formation and the expression and retention of bladder smooth muscle contractile proteins of human derivation. Quantitative morphometry of MSC/UC samples revealed muscle/collagen ratios approximately 1.75x greater than SMC/UC controls at 10 weeks. Data demonstrate MSC seeded POCfs support partial regeneration of bladder tissue in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Azo Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Citrates,
http://linkedlifedata.com/resource/pubmed/chemical/Citric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Elastomers,
http://linkedlifedata.com/resource/pubmed/chemical/Eosine Yellowish-(YS),
http://linkedlifedata.com/resource/pubmed/chemical/Methyl Green,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/poly(1,8-octanediol citrate),
http://linkedlifedata.com/resource/pubmed/chemical/trichrome stain
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
1878-5905
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| pubmed:author | |
| pubmed:copyrightInfo |
2010 Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
31
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6207-17
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| pubmed:meshHeading |
pubmed-meshheading:20488535-Animals,
pubmed-meshheading:20488535-Azo Compounds,
pubmed-meshheading:20488535-Bone Marrow Cells,
pubmed-meshheading:20488535-Cell Survival,
pubmed-meshheading:20488535-Citrates,
pubmed-meshheading:20488535-Citric Acid,
pubmed-meshheading:20488535-Collagen,
pubmed-meshheading:20488535-Elastic Modulus,
pubmed-meshheading:20488535-Elastomers,
pubmed-meshheading:20488535-Eosine Yellowish-(YS),
pubmed-meshheading:20488535-Female,
pubmed-meshheading:20488535-Fluorescent Antibody Technique,
pubmed-meshheading:20488535-Humans,
pubmed-meshheading:20488535-Mesenchymal Stem Cells,
pubmed-meshheading:20488535-Methyl Green,
pubmed-meshheading:20488535-Muscle, Smooth,
pubmed-meshheading:20488535-Polymers,
pubmed-meshheading:20488535-Rats,
pubmed-meshheading:20488535-Rats, Nude,
pubmed-meshheading:20488535-Regeneration,
pubmed-meshheading:20488535-Staining and Labeling,
pubmed-meshheading:20488535-Tissue Scaffolds,
pubmed-meshheading:20488535-Urinary Bladder
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| pubmed:year |
2010
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| pubmed:articleTitle |
Urinary bladder smooth muscle regeneration utilizing bone marrow derived mesenchymal stem cell seeded elastomeric poly(1,8-octanediol-co-citrate) based thin films.
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| pubmed:affiliation |
Children's Memorial Hospital Chicago, Division of Pediatric Urology, 2300 Children's Plaza, Chicago, IL 60614, USA. arun-sharma@northwestern.edu
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| pubmed:publicationType |
Journal Article
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