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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2010-7-14
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pubmed:abstractText |
TANK/I-TRAF is a TRAF-binding protein that negatively regulates NF-kappaB activation. The underlying mechanism of this activity remains unclear. Here we show that TANK directly interacts with PLK1, a conserved cell cycle-regulated kinase. PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha, IL-1beta, or several activators, but not by nuclear transcription factor p65. PLK1 expression reduces the DNA-binding activity of NF-kappaB induced by TNF-alpha. Moreover, endogenous activation of PLK1 reduces the TNF-induced phosphorylation of endogenous IkappaBalpha. PLK1 is bound to NEMO (IKKgamma) through TANK to form a ternary complex in vivo. We describe a new regulatory mechanism for PLK1: PLK1 negatively regulates TNF-induced IKK activation by inhibiting the ubiquitination of NEMO. These findings reveal that the scaffold protein TANK recruits PLK1 to negatively regulate NF-kappaB activation and provide direct evidence that PLK1 is required for the repression function of TANK.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IKBKG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TANK protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TBK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/polo-like kinase 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1939-4586
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2500-13
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:20484576-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:20484576-Cell Cycle,
pubmed-meshheading:20484576-Cell Cycle Proteins,
pubmed-meshheading:20484576-Cell Line,
pubmed-meshheading:20484576-Cytoplasm,
pubmed-meshheading:20484576-Enzyme Activation,
pubmed-meshheading:20484576-Humans,
pubmed-meshheading:20484576-I-kappa B Kinase,
pubmed-meshheading:20484576-I-kappa B Proteins,
pubmed-meshheading:20484576-Mutation,
pubmed-meshheading:20484576-NF-kappa B,
pubmed-meshheading:20484576-Phosphorylation,
pubmed-meshheading:20484576-Protein Binding,
pubmed-meshheading:20484576-Protein Interaction Mapping,
pubmed-meshheading:20484576-Protein Processing, Post-Translational,
pubmed-meshheading:20484576-Protein Transport,
pubmed-meshheading:20484576-Protein-Serine-Threonine Kinases,
pubmed-meshheading:20484576-Proto-Oncogene Proteins,
pubmed-meshheading:20484576-Transcriptional Activation,
pubmed-meshheading:20484576-Tumor Necrosis Factor-alpha,
pubmed-meshheading:20484576-Two-Hybrid System Techniques,
pubmed-meshheading:20484576-Ubiquitination
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pubmed:year |
2010
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pubmed:articleTitle |
The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1.
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pubmed:affiliation |
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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