Linked Life Data

20483487

Source:http://linkedlifedata.com/resource/pubmed/id/20483487

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-8-16
pubmed:abstractText
The immunoglobulin heavy chain variable region in vertebrates is composed of many genes. However, the gene number and the extent of diversity among the VH gene segments vary with the species. To gain insight into the dog VH repertoire, we analyzed its genome sequence. Eighty VH, six DH and three JH segments were mapped to a 1.28Mb region of the dog chromosome 8. Forty-one of these VH genes were potentially functional, and 39 were pseudogenes. The sequence similarity analysis suggests that the dog IgH locus harbors three VH gene families, VH1 (including 76 members), VH2 (including 3 members) and VH3 (including one member), that are classified into mammalian clan III, I and II, respectively. Subsequently, 111 cDNA positive clones from dog 26 mu, 22 gamma, 23 alpha, 16 delta and 24 epsilon chains containing almost full-length VH, DH and JH segments were selected at random. All of the sequences, except for clone G6, which belonged to the dog VH2 family, originated from the dog VH1 family. The germline segments, VH62 (human VH3-21 homology) and VH44 (human VH3-23 homology), seemed to be preferentially used. Our results suggest that the dog VH repertoire appears to be derived from limited germline gene families, and its diversity may rely on junctional diversity and somatic hypermutation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1873-2534
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
64-75
pubmed:dateRevised
2010-12-31
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Molecular characterization of the VH repertoire in Canis familiaris.
pubmed:affiliation
College of Animal Science and Veterinary Medicine, Agricultural Division, Jilin University, 5333 of Xi'an Road, Changchun, Jilin 130062, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't