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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-5-20
pubmed:abstractText
In insects, chitinases play an essential role in the degradation of old exoskeleton and turnover of the gut lining. In silico screening of the entire genome of the pea aphid (Hemimetabola), Acyrthosiphon pisum, detected nine genes encoding putative chitinase-like proteins, including six enzymatically active chitinases, one imaginal disc growth factor, and one endo-beta-N-acetylglucosaminidase. Screening of the genomes of Aedes aegypti, Anopheles gambiae, Apis mellifera, Bombyx mori, Culex quinquefasciatus, Drosophila melanogaster, Nasonia vitripennis, Pediculus humanus corporis, and Tribolium castaneum suggested repeated gene duplications in holometabolous lineages. Quantitative reverse transcription-PCR demonstrated the expression of four and two distinct chitinase-like genes of A. pisum to be highly up-regulated in the embryo and the midgut, respectively, suggesting specific roles in these pea aphid tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1365-2583
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19 Suppl 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-85
pubmed:meshHeading
pubmed-meshheading:20482649-Amino Acid Sequence, pubmed-meshheading:20482649-Animals, pubmed-meshheading:20482649-Aphids, pubmed-meshheading:20482649-Base Sequence, pubmed-meshheading:20482649-Chitinase, pubmed-meshheading:20482649-DNA Primers, pubmed-meshheading:20482649-Evolution, Molecular, pubmed-meshheading:20482649-Gene Duplication, pubmed-meshheading:20482649-Gene Expression, pubmed-meshheading:20482649-Gene Expression Profiling, pubmed-meshheading:20482649-Genome, Insect, pubmed-meshheading:20482649-Insect Proteins, pubmed-meshheading:20482649-Insects, pubmed-meshheading:20482649-Molecular Sequence Data, pubmed-meshheading:20482649-Peas, pubmed-meshheading:20482649-Phylogeny, pubmed-meshheading:20482649-Sequence Homology, Amino Acid, pubmed-meshheading:20482649-Species Specificity
pubmed:year
2010
pubmed:articleTitle
Chitinase-like proteins encoded in the genome of the pea aphid, Acyrthosiphon pisum.
pubmed:affiliation
Advanced Science Institute, RIKEN, Wako, Saitama, Japan. bachi@riken.jp <bachi@riken.jp>
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't