Linked Life Data

20482637

Source:http://linkedlifedata.com/resource/pubmed/id/20482637

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-5-20
pubmed:abstractText
Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5' and 3' ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1365-2583
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19 Suppl 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-31
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A full-length cDNA resource for the pea aphid, Acyrthosiphon pisum.
pubmed:affiliation
HHMI and Department of Ecology and Evolutionary Biology, Princeton University, NJ, USA. shige@nibb.ac.jp <shige@nibb.ac.jp>
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't