Linked Life Data

20478639

Source:http://linkedlifedata.com/resource/pubmed/id/20478639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-10-18
pubmed:abstractText
Accurate measurements of dissolved O(2) as a function of time have numerous chemical and biological applications. The Pd (II) complex of meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin (Pd phosphor) was used for this purpose. Detection is based on the principle that the phosphorescence of this oxygen probe is inversely related to dissolved O(2) (O(2) quenches the phosphorescence). Biologic samples containing the Pd phosphor were flashed (10/s) with a peak output of 625nm; emitted light was detected at 800nm. Amplified pulses of phosphorescence were digitized at 1-2MHz using an analog/digital converter (PCI-DAS 4020/12 I/O Board) with outputs ranging from 1 to 20MHz. Assessment revealed a customized program was necessary. Pulses were captured using a developed software at 0.1-4MHz, depending on the speed of the computer. O(2) concentration was calculated by fitting to an exponential the decay of the phosphorescence. Twelve tasks were identified, which allowed full control and customization of the data acquisition, storage and analysis. The program used Microsoft Visual Basic 6 (VB6), Microsoft Access Database 2007, and a Universal Library component that allowed direct reading from the PCI-DAS 4020/12 I/O Board. It involved a relational database design to store experiments, pulses and pulse metadata, including phosphorescence decay rates. The method permitted reliable measurements of cellular O(2) consumption over several hours.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1872-7565
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
265-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Oxygen measurements via phosphorescence.
pubmed:affiliation
Department of Medical Education, United Arab Emirates University, Faculty of Medicine and Health Sciences, Al Ain, United Arab Emirates. sami.shaban@uaeu.ac.ae
pubmed:publicationType
Journal Article