Source:http://linkedlifedata.com/resource/pubmed/id/20473534
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-10-26
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| pubmed:abstractText |
Anti-bacterial drug resistance is one of the most critical concerns among the scientist worldwide. The novel antimicrobial decapeptide SD-8 is designed and its minimal inhibitory concentration and therapeutic index (TI) was found in the range of 1-8 ?g/ml and 45-360, respectively, against major group of Gram positive pathogens (GPP). The peptide was also found to be least hemolytic at a concentration of 180 ?g/ml, i.e., nearly 77 times higher than its average effective concentration. The kinetics assay showed that the killing time is 120 min for methicillin-sensitive Staphylococcus aureus (MSSA) and 90 min for methicillin-resistant S. aureus (MRSA). Membrane permeabilization is the cause of peptide antimicrobial activity as shown by the transmission electron microscopy studies. The peptide showed the anti-inflammatory property by inhibiting COX-2 with a KD and Ki values of 2.36×10(-9) and 4.8×10(-8) M, respectively. The peptide was also found to be effective in vivo as derived from histopathological observations in a Staphylococcal skin infection rat model with MRSA as causative organism.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1438-2199
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
39
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1493-505
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| pubmed:meshHeading |
pubmed-meshheading:20473534-Animals,
pubmed-meshheading:20473534-Anti-Bacterial Agents,
pubmed-meshheading:20473534-Antimicrobial Cationic Peptides,
pubmed-meshheading:20473534-Chromatography, High Pressure Liquid,
pubmed-meshheading:20473534-Cyclooxygenase 2,
pubmed-meshheading:20473534-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:20473534-Disease Models, Animal,
pubmed-meshheading:20473534-Drug Resistance, Multiple,
pubmed-meshheading:20473534-Gram-Positive Cocci,
pubmed-meshheading:20473534-Hemolysis,
pubmed-meshheading:20473534-Humans,
pubmed-meshheading:20473534-Kinetics,
pubmed-meshheading:20473534-Microbial Sensitivity Tests,
pubmed-meshheading:20473534-Peptides,
pubmed-meshheading:20473534-Rats,
pubmed-meshheading:20473534-Rats, Wistar,
pubmed-meshheading:20473534-Skin,
pubmed-meshheading:20473534-Solubility,
pubmed-meshheading:20473534-Surface Plasmon Resonance
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| pubmed:year |
2010
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| pubmed:articleTitle |
SD-8, a novel therapeutic agent active against multidrug-resistant Gram positive cocci.
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| pubmed:affiliation |
Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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