Linked Life Data

20469649

Source:http://linkedlifedata.com/resource/pubmed/id/20469649

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2010-5-17
pubmed:abstractText
Imaginal disc growth factors (IDGF) play a key role in insect development, but their mechanism remains unclear. In this study, we cloned a novel IDGF gene in Bombyx mori and designated it as BmlDGF. We found that the BmlDGF gene contains eight exons and seven introns, encoding a peptide of 434 amino-acid residues. The protein was predicted to contain one conserved motif of the glycosyl hydrolases family 18 and fall into group V chitinases. Sequence alignment showed that BmIDGF shares extensive homology with other invertebrate IDGF. RT-PCR analysis showed that BmIDGF is expressed in all developmental stages of silkworm larvae and various larvae tissues, which was further confirmed by Western blot analysis. Subcellular localization analysis indicated that BmIDGF is located in the extracellular space. We also successfully expressed it in E. coli and further characterized it by SDS-PAGE and mass spectrometry. Taken together, our data suggests that BmIDGF is a chitinase-like extracellular protein, and provides an excellent platform for subsequent studies on its enzyme activity and role in B. mori development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0939-5075
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-83
pubmed:meshHeading
pubmed:articleTitle
Molecular cloning, expression and characterization of BmIDGF gene from Bombyx mori.
pubmed:affiliation
Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't