Linked Life Data

20461753

Source:http://linkedlifedata.com/resource/pubmed/id/20461753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-5-12
pubmed:abstractText
Karyotypic analysis and genomic copy number analysis with single nucleotide polymorphism (SNP)-based microarrays were compared with regard to the detection of recurrent genomic imbalances in 20 clear cell renal cell carcinomas (ccRCCs). Genomic imbalances were identified in 19 of 20 tumors by DNA copy number analysis and in 15 tumors by classical cytogenetics. A statistically significant correlation was observed between the number of genomic imbalances and tumor stage. The most common genomic imbalances were loss of 3p and gain of 5q. Other recurrent genomic imbalances seen in at least 15% of tumors included losses of 1p32.3-p33, 6q23.1-qter and 14q and gain of chromosome 7. The SNP-based arrays revealed losses of 3p in 16 of 20 tumors, with the highest frequency being at 3p21.31-p22.1 and 3p24.3-p25.3, the latter encompassing the VHL locus. One other tumor showed uniparental disomy of chromosome 3. Thus, altogether loss of 3p was identified in 17 of 20 (85%) cases. Fourteen tumors showed both overlapping losses of 3p and overlapping gains of 5q, and the karyotypic assessment performed in parallel revealed that these imbalances arose via unbalanced 3;5 translocations. Among the latter, there were common regions of loss at 3p21.3-pter and gain at 5q34-qter. These data suggest that DNA copy number analysis will supplant karyotypic analysis of tumor types such as ccRCC that are characterized by recurrent genomic imbalances, rather than balanced rearrangements. These findings also suggest that the 5q duplication/3p deficiency resulting from unbalanced 3;5 translocations conveys a proliferative advantage of particular importance in ccRCC tumorigenesis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-11691785, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-11921283, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-12953746, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-15809763, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-15949569, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-17922474, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-17968032, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-18194544, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-18359287, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-18592004, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-18682315, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-19124809, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-19470766, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-2176544, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-3610386, http://linkedlifedata.com/resource/pubmed/commentcorrection/20461753-7915601
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1098-2264
pubmed:author
pubmed:copyrightInfo
(c) 2010 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
610-9
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Combined classical cytogenetics and microarray-based genomic copy number analysis reveal frequent 3;5 rearrangements in clear cell renal cell carcinoma.
pubmed:affiliation
Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural