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rdf:type |
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lifeskim:mentions |
umls-concept:C0003402,
umls-concept:C0017817,
umls-concept:C0033268,
umls-concept:C0085403,
umls-concept:C0127400,
umls-concept:C0141729,
umls-concept:C0205332,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1705920,
umls-concept:C1706817,
umls-concept:C1882687,
umls-concept:C2911692
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pubmed:issue |
5
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pubmed:dateCreated |
2010-5-12
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pubmed:abstractText |
To investigate the involvement of reactive oxidant species (ROS), presumably arising from cytochrome P-450 (CYP)-catalyzed metabolism of schisandrin B (Sch B), in triggering glutathione antioxidant response, Sch B induced reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent and CYP-catalyzed reaction and associated ROS production were examined in rat heart microsomes. Sch B analogs were also studied for comparison. Using rat heart microsomes as a source of CYP, Sch B and schisandrin C (Sch C), but not schisandrin A and dimethyl diphenyl bicarboxylate (an intermediate compound derived from the synthesis of Sch C), were found to serve as co-substrate for the CYP-catalyzed NADPH oxidation reaction, with concomitant production of ROS. The stimulation of CYP-catalyzed NADPH oxidation reaction and/or ROS production by Sch B or Sch C correlated with the increase in mitochondrial reduced glutathione level and protection against ischemia/reperfusion (I/R) injury in rat hearts. The involvement of ROS in Sch B-induced cardioprotection was further confirmed by the suppressive effect produced by N-acetylcysteine or alpha-tocopherol pretreatment. Taken together, these results suggest that Sch B-induced glutathione antioxidant response and cardioprotection may be mediated by ROS arising from CYP-catalyzed reaction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiovascular Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooctanes,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Lignans,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/Polycyclic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Tocopherol,
http://linkedlifedata.com/resource/pubmed/chemical/schizandrin B,
http://linkedlifedata.com/resource/pubmed/chemical/schizandrin C
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pubmed:status |
MEDLINE
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pubmed:issn |
1347-5215
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
825-9
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pubmed:meshHeading |
pubmed-meshheading:20460761-Acetylcysteine,
pubmed-meshheading:20460761-Animals,
pubmed-meshheading:20460761-Antioxidants,
pubmed-meshheading:20460761-Cardiovascular Agents,
pubmed-meshheading:20460761-Cyclooctanes,
pubmed-meshheading:20460761-Cytochrome P-450 Enzyme System,
pubmed-meshheading:20460761-Drugs, Chinese Herbal,
pubmed-meshheading:20460761-Female,
pubmed-meshheading:20460761-Glutathione,
pubmed-meshheading:20460761-Lignans,
pubmed-meshheading:20460761-Microsomes,
pubmed-meshheading:20460761-Mitochondria,
pubmed-meshheading:20460761-Myocardial Ischemia,
pubmed-meshheading:20460761-Myocardial Reperfusion Injury,
pubmed-meshheading:20460761-Myocardium,
pubmed-meshheading:20460761-NADP,
pubmed-meshheading:20460761-Oxidation-Reduction,
pubmed-meshheading:20460761-Phytotherapy,
pubmed-meshheading:20460761-Polycyclic Compounds,
pubmed-meshheading:20460761-Rats,
pubmed-meshheading:20460761-Rats, Sprague-Dawley,
pubmed-meshheading:20460761-Reactive Oxygen Species,
pubmed-meshheading:20460761-Schisandra,
pubmed-meshheading:20460761-alpha-Tocopherol
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pubmed:year |
2010
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pubmed:articleTitle |
Schisandrin B-induced glutathione antioxidant response and cardioprotection are mediated by reactive oxidant species production in rat hearts.
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pubmed:affiliation |
Department of Biochemistry, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong SAR, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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