Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2010-7-12
pubmed:abstractText
We identified human phosphatidylethanolamine-binding protein 4 (hPEBP4) as a human-derived novel member of the phosphatidylethanolamine-binding protein family, which is involved in apoptosis resistance of tumor cells. Because of its preferential expression in estrogen-related cancers, we wondered whether hPEBP4 plays a role in estrogen-induced cancer cell growth. Here, we demonstrated that hPEBP4 inhibited the 17beta-estradiol (E(2))-induced, proteasome-dependent estrogen receptor alpha (ERalpha) degradation to increase the protein level of ERalpha. Silencing of hPEBP4 inhibited the recruitment of ERalpha to the promoter of the ERalpha target gene pS2 in MCF-7 breast cancer cells after E(2) treatment. E(2)-induced, ERalpha-mediated transcription via the estrogen-response element, as well as the cellular proliferation, was significantly suppressed in hPEBP4-silenced MCF-7 cells. We found that Src, whose association with ERalpha facilitates the ERalpha binding to components of proteolytic machinery, could associate with hPEBP4 and that overexpression of hPEBP4 prevented the E(2)-induced interaction between ERalpha and Src. ERalpha overexpression, proteasome inhibitor, or Src inhibitor could reverse the suppression of ERalpha-mediated transactivation by hPEBP4 silencing. The inhibition of the proteasome degradation and the promotion of transactivation of ERalpha by hPEBP4 via the Src pathway were further confirmed in HeLa cells. Finally, we found that the promoting effects of hPEBP4 on ERalpha-mediated transactivation and estrogen-induced proliferation of cancer cells did not depend on its regulation of Akt and ERK activity. Our data suggest that hPEBP4 inhibits proteasome-dependent ERalpha degradation through the Src pathway, thus enhancing ERalpha-mediated transactivation and promoting the proliferation of cancer cells in response to estrogen.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-10334989, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-10478843, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-10911988, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-10953052, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-10976921, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-11162945, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-11178989, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-11459779, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-11509655, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-12198236, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-12461787, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-12754377, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-12855746, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-12865327, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-14718389, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-14973112, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15284335, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15302887, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15528270, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-1569962, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15701888, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15705661, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15709962, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-15899841, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16084012, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16168139, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16243830, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16293641, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16421368, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-16865237, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17157789, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17178731, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17440111, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17615392, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17627304, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-17699868, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-18305109, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-18818394, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-19056740, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-19483718, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-19707857, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-7539106, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-7979245, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-8635462, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-8994187, http://linkedlifedata.com/resource/pubmed/commentcorrection/20460377-9442882
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/4-amino-5-(4-methylphenyl)-7-(tert-b..., http://linkedlifedata.com/resource/pubmed/chemical/Estradiol, http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor alpha, http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP..., http://linkedlifedata.com/resource/pubmed/chemical/PE-binding protein 4, human, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamine Binding..., http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt, http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles, http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines, http://linkedlifedata.com/resource/pubmed/chemical/estrogen receptor alpha, human, http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1083-351X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
16
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21934-42
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
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