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rdf:type |
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lifeskim:mentions |
umls-concept:C0004199,
umls-concept:C0007634,
umls-concept:C0011860,
umls-concept:C0021641,
umls-concept:C0021670,
umls-concept:C0022131,
umls-concept:C0026336,
umls-concept:C0030685,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0040676,
umls-concept:C0075444,
umls-concept:C0391871,
umls-concept:C0441994,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1412125,
umls-concept:C1538557,
umls-concept:C1963578
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pubmed:issue |
1-2
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pubmed:dateCreated |
2010-6-14
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pubmed:abstractText |
Succinyl-CoA:3-ketoacid-CoA transferase (SCOT) is a mitochondrial enzyme that catalyzes the reversible transfer of coenzyme-A from acetoacetyl-CoA to succinate to form acetoacetate and succinyl-CoA. mRNAs of SCOT and ATP citrate lyase were decreased 55% and 58% and enzyme activities were decreased >70% in pancreatic islets of the GK rat, a model of type 2 diabetes. INS-1 832/13 cells were transfected with shRNAs targeting SCOT mRNA to generate cell lines with reduced SCOT activity. Two cell lines with >70% knockdown of SCOT activity showed >70% reduction in glucose- or methyl succinate-plus-beta-hydroxybutyrate-stimulated insulin release. Less inhibition of insulin release was observed with two cell lines with less knockdown of SCOT. Previous studies showed knockdown of ATP citrate lyase in INS-1 832/13 cells does not lower insulin release. The results further support work that suggests mitochondrial pathways involving SCOT which supply acetoacetate for export to the cytosol are important for insulin secretion.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-10868964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-10905479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-12086928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-12093799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-1370150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-16740637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-17102138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-17724028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-17823126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-17928289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18160486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-1825072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18414997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18439432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18697738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18755687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18769905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-18802677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-19296078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-19691144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-19858194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-6462111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-6790537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-7650022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8163052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8373157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8405739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8412734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8424653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8576218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8666138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-8866570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-9228023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20460097-9974390
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1096-0384
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
499
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
62-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20460097-Animals,
pubmed-meshheading:20460097-Rats,
pubmed-meshheading:20460097-Insulin,
pubmed-meshheading:20460097-Male,
pubmed-meshheading:20460097-Mitochondria,
pubmed-meshheading:20460097-Islets of Langerhans,
pubmed-meshheading:20460097-Base Sequence,
pubmed-meshheading:20460097-Disease Models, Animal,
pubmed-meshheading:20460097-Rats, Wistar,
pubmed-meshheading:20460097-RNA, Messenger,
pubmed-meshheading:20460097-Models, Biological,
pubmed-meshheading:20460097-Cell Line,
pubmed-meshheading:20460097-Diabetes Mellitus, Type 2
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