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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-5-20
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pubmed:abstractText |
Lemay et al recently reported that the RNA binding protein HuR directly interacts with the ribonuclease H (RNase H) domain of HIV-1 reverse transcriptase (RT) and influences the efficiency of viral reverse transcription (Lemay et al., 2008, Retrovirology 5:47). HuR is a member of the embryonic lethal abnormal vision protein family and contains 3 RNA recognition motifs (RRMs) that bind AU-rich elements (AREs). To define the structural determinants of the HuR-RT interaction and to elucidate the mechanism(s) by which HuR influences HIV-1 reverse transcription activity in vitro, we cloned and purified full-length HuR as well as three additional protein constructs that contained the N-terminal and internal RRMs, the internal and C-terminal RRMs, or the C-terminal RRM only.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-10490959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-1280240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-1377403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-14981256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-15457527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-15970587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-16782553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-1688798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-17517897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-17632515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18045931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18187620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18544151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18593881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18786546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18854154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18948743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-18976975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-19067547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-19246451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-1984479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-7541845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-7898713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-8594344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-8626503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-8970997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-9135139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-9261439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-9557701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20459669-9971795
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/ELAVL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Reverse Transcriptase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/reverse transcriptase, Human...
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pubmed:status |
MEDLINE
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pubmed:issn |
1742-4690
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
40
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20459669-Antigens, Surface,
pubmed-meshheading:20459669-Cloning, Molecular,
pubmed-meshheading:20459669-HIV Reverse Transcriptase,
pubmed-meshheading:20459669-HIV-1,
pubmed-meshheading:20459669-Humans,
pubmed-meshheading:20459669-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:20459669-Protein Interaction Mapping,
pubmed-meshheading:20459669-Protein Multimerization,
pubmed-meshheading:20459669-RNA, Viral,
pubmed-meshheading:20459669-RNA-Binding Proteins,
pubmed-meshheading:20459669-Recombinant Proteins,
pubmed-meshheading:20459669-Reverse Transcription
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pubmed:year |
2010
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pubmed:articleTitle |
The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro.
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pubmed:affiliation |
Department of Structural Biology, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. jia12@pitt.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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