Linked Life Data

20457547

Source:http://linkedlifedata.com/resource/pubmed/id/20457547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2010-5-31
pubmed:abstractText
A rapid, specific, and sensitive method utilizing reversed-phase ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated to determine finasteride levels in human plasma. The plasma samples were prepared by liquid-liquid extraction with ethyl acetate, evaporation, and reconstitution. MS/MS analyses were performed on a triple-quadrupole tandem mass spectrometer by monitoring protonated parent-->daughter ion pairs at m/z 373-->305 for finasteride and m/z 237-->194 for carbamazepine (internal standard, IS). The method was validated with respect to linearity, recovery, specificity, accuracy, precision, and stability. The method exhibited a linear response from 0.1 to 30 ng/mL (r(2)>0.998). The limit of quantitation for finasteride in plasma was 0.1 ng/mL. The relative standard deviation (RSD) of intra- and inter-day measurements was less than 15% and the method was accurate within -6.0% to 2.31% at all quality-control levels. The mean extraction recovery was higher than 83% for finasteride and 84% for the IS. Plasma samples containing finasteride were stable under the three sets of conditions tested and the processed samples were stable up to 29 h in an autosampler at 5 degrees C. Detection and quantitation of both analytes within 3 min make this method suitable for high-throughput analyses. The method was successfully applied to a pharmacokinetic study of finasteride in healthy volunteers following oral administration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-376X
pubmed:author
pubmed:copyrightInfo
2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
878
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1718-23
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Rapid determination of finasteride in human plasma by UPLC-MS/MS and its application to clinical pharmacokinetic study.
pubmed:affiliation
Department of Molecular Medicine, Kyungpook National University School of Medicine, Daegu, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies