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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2010-7-16
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pubmed:abstractText |
Mucosal delivery of peptide/protein therapeutics via the oral route is a desirable strategy in human immunotherapy. A key step for enhancing the bioavailability of orally administered therapeutics is to protect them from enzymatic digestion in the gastrointestinal tract. Here, we generated transgenic rice seeds accumulating allergen-derived T cell epitopes, a model tolerogen for the control of pollen allergy, in either ER-derived protein body-I (PB-I) or protein storage vacuole protein body-II (PB-II). Compared with PB-II-localized or chemically synthesized forms, PB-I-localized T cell epitopes showed higher resistance to enzymatic digestion in simulated gastric fluid. Moreover, the dose of T cell epitope required for suppression of allergen-specific IgE in mice was about 20-fold lower when fed in PB-I localized form than when unprotected. These findings demonstrate the potential of bioencapsulation in PB-I for broad applications as a viable strategy to achieve efficient mucosal delivery of oral peptide/protein therapeutics.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/Cry j I protein, Cryptomeria...,
http://linkedlifedata.com/resource/pubmed/chemical/Cry j II protein, Cryptomeria...,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1873-5169
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1421-5
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pubmed:meshHeading |
pubmed-meshheading:20457197-Administration, Oral,
pubmed-meshheading:20457197-Animals,
pubmed-meshheading:20457197-Antigens, Plant,
pubmed-meshheading:20457197-Cytoplasmic Vesicles,
pubmed-meshheading:20457197-Dose-Response Relationship, Immunologic,
pubmed-meshheading:20457197-Drug Delivery Systems,
pubmed-meshheading:20457197-Endoplasmic Reticulum,
pubmed-meshheading:20457197-Epitopes, T-Lymphocyte,
pubmed-meshheading:20457197-Gastric Juice,
pubmed-meshheading:20457197-Hypersensitivity,
pubmed-meshheading:20457197-Immunoglobulin E,
pubmed-meshheading:20457197-Immunosuppression,
pubmed-meshheading:20457197-Male,
pubmed-meshheading:20457197-Mice,
pubmed-meshheading:20457197-Mice, Inbred BALB C,
pubmed-meshheading:20457197-Oryza sativa,
pubmed-meshheading:20457197-Peptides,
pubmed-meshheading:20457197-Plant Proteins,
pubmed-meshheading:20457197-Plants, Genetically Modified,
pubmed-meshheading:20457197-Pollen,
pubmed-meshheading:20457197-Recombinant Fusion Proteins,
pubmed-meshheading:20457197-Seeds
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pubmed:year |
2010
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pubmed:articleTitle |
Rice seed ER-derived protein body as an efficient delivery vehicle for oral tolerogenic peptides.
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pubmed:affiliation |
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. hitakagi@affrc.go.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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